duoset elisa development systems  (R&D Systems)

Journal: Parasite Immunology

Article Title: The Regulatory Effect of Histone Deacetylase ( HDAC ) 1 and 2 on iNOS , IL ‐6, TNF ‐α and IL ‐10 Expression in Canine Macrophages Infected With Leishmania infantum

doi: 10.1111/pim.70078

Figure Lengend Snippet: Inhibition of HDAC1 in cells infected with Leishmania infantum and treated with sodium butyrate on IL‐6, TNF‐α and IL‐10 expression. DH82 cells were treated with the pharmacological HDAC1 inhibitor sodium butyrate NaB (10 mM) and (20 mM). After 6 h of infection, the culture supernatant cells were harvested, and (A) IL‐6, (B) TNF‐α and (C) IL‐10 were measured by capture ELISA. The statistical test used was the Friedman test, followed by Dunn's multiple‐comparison test ( p < 0.05).

Article Snippet: Cytokines IL‐6, TNF‐α and IL‐10 were quantified in culture supernatant from L. infantum infected macrophages after HDAC1 and HDAC2 suppression with NaB (10 mM) and (20 mM) (Sigma‐Aldrich, MO, USA) by using the DuoSet ELISA Development Systems canine kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's instructions.

Techniques: Inhibition, Infection, Expressing, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Frontiers in Immunology

Article Title: Phosphatidylserine liposomes for Mycobacterium abscessus infections management in people with cystic fibrosis non-eligible for CFTR modulators

doi: 10.3389/fimmu.2026.1681558

Figure Lengend Snippet: PS-L beneficially modulates pro- and anti-inflammatory cytokines IL-1β, TNF-α, and IL-10 production over the course of Mab infection on pwCF macrophages. MDM from pwCF ( n = 6) were cultured at the concentration of 1x10 6 cells/ml in 96-well plates. Cells were infected or not with Mab at MOI 10 for 3 hours at 37°C and then extracellular bacilli were killed by 1 hour incubation with amikacin 250µg/ml. Cells were finally stimulated or not with PS-L for 3 (A, C, E) or 18 hours (B, D, F) and supernatants harvested and stored at -20 °C until analysis. The production of IL-1β, TNF-α, and IL-10 was analyzed by ELISA as per manufacturer’s instructions. Baseline cytokines range levels from uninfected and untreated controls are displayed in the background as mean ± 95% CI. Statistical analysis was performed by using two-sided Wilcoxon matched-pairs signed rank test. ns = not significant; *p<0.05.

Article Snippet: The levels of TNF-α, IL-1β, and IL-10 were measured by human TNF-α, IL-1β, or IL-10 DuoSet ® ELISA Development Systems (R&D Systems, Minneapolis, MN, USA) as per manufacturer’s instructions.

Techniques: Infection, Cell Culture, Concentration Assay, Incubation, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Temporal Dynamics of Kidney Mitochondrial Dysfunction in Type 2 Diabetes: Analysis of the Goto-Kakizaki Model

doi: 10.64898/2025.12.27.696702

Figure Lengend Snippet: Cytokine levels in the kidneys of GK and Wistar rats over time assessed by ELISA. (A) IL-1β levels showing no significant differences across the groups. (B) IL-18 levels with a significant increase in the GK21D group compared to all other groups. (C) CXCL1 levels with a significant increase in the GK21D group compared to all other groups. (D) IL-10 levels showing significantly elevated concentrations in the GK21D and GK60D groups compared to the GK120D group. Data are expressed as percentage relative to the control group and presented as mean ± standard error of the mean (SEM).

Article Snippet: Relative levels of CXCL1, IL-10, IL-1β, and IL-18 in renal tissue were assessed by sandwich ELISA using the DuoSet® ELISA Development System (R&D Systems, Minneapolis, MN, USA; catalog numbers DY275, DY217B, DY201, and DY318).

Techniques: Enzyme-linked Immunosorbent Assay, Control